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SCRIPT RT-qPCR SybrMaster highROX (2x conc.), 2 x 1.25 ml, Jena Bioscience

447,90 €
SKU
Jena-Bioscience-PCR-522S
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RT-real-time-PCR mix with SYBR® Green fluorescent DNA stain and highROX

SCRIPT RT-qPCR SybrMaster highROX is designed for quantitative real-time analyses of RNA templates using the fluorescent DNA stain SYBR® Green. The ready-to-use mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.
The 2x conc. mix contains all reagents required for RT-qPCR (except template and primers) allow fast and easy preparation with a minimum of pipetting steps. The premium quality enzymes and the optimized reaction buffer ensure superior real time PCR results.
RT-qPCR is used to amplify double-stranded DNA from single-stranded RNA templates to allow a rapid real-time quantification of RNA targets. In the reverse transcription step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. In the first cycle of the PCR step the hot-start DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. The hot-start polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
One-step RT-qPCR offers tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.

ROX reference dye:
The SCRIPT RT-qPCR SybrMaster highROX contains 500 nM ROX passive reference dye in the final assay. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.

Content:
SCRIPT RT-qPCR SybrMaster lowROX
Ready-to-use mix of SCRIPT Reverse Transcriptase, Hot Start Polymerase, RNase Inhibitor, dNTPs, reaction buffer, SybrGreen fluorescent DNA stain, ROX and stabilizers.
PCR-grade Water
SYBR® Green fluorescent DNA stain:
SYBR® Green fluorescent DNA stain is a superior DNA intercalator dye specially developed for DNA analysis applications like real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing only lowest inhibition to the PCR process. The dye is stable both thermally and hydrolytically, providing convenience during routine handling.
Sensitivity:
Targets can generally be detected from <1 pg to 20 ng poly(A) RNA (mRNA) or 10 pg to 1 μg total RNA. Even lower amounts of RNA may be successfully amplified by using highly expressed transcripts.


For general laboratory use.

Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark
stable at 4 °C for up to 4 weeks
Shelf Life: 12 months
Form: liquid
Concentration: 2x conc.
Spectroscopic Properties: λexc 494 nm (bound to DNA), λem 521 nm (bound to DNA)

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